固绿FCF/ 坚牢绿FCF/快绿/Fast green FCF,≥90%
- 产品编号:F0209
- 中文名称:固绿FCF/ 坚牢绿FCF/快绿/Fast green FCF
- 英文名称:Fast green FCF
- 英文别名:Food green 3
- CAS号:2353-45-9
- 分子式:C37H34N2O10S3Na2
- 分子量:808.85
- 纯度:≥90%
- EC号:219-091-5
- MDL号:MFCD00013053
性能描述
composition dye content, ≥90%
mp 290 °C (dec.)(lit.)
solubility H2O: soluble1 mg/mL
Application
Fast Green FCF dye is used for protein staining in IEF, native PAGE and SDS-PAGE. Fast Green staining is linear over a wider range of protein concentrations than Brilliant Blue R. After electrophoresis, fixing the proteins in the gel is recommended for maximum sensitivity. The gel can then be stained for 2 hr with 0.1% Fast Green FCF in either 30% (v/v) ethanol, 10% (v/v) acetic acid or in 7% acetic acid. The gel can be destained with either 30% (v/v) ethanol, 10% (v/v) acetic acid, or 7% acetic acid. The stained gel can be scanned at 625 nm. The detection sensitivity is approx. 30% that with Brilliant Blue R.
溶解性:有吸湿性。易溶于水,溶于乙醇,溶于浓硫酸呈暗橙色,稀释后呈暗绿色,溶于浓盐酸或浓硝酸呈橙色,溶于10%氢氧化钠溶液呈亮蓝色。
Suitability
Certified for the safranin-fast green FCF stain for plant cells; as a substitute for aniline blue in Gomori′s one-step trichrome procedure.