性能描述

assay    ≥99% (HPLC) 
form    solid 
color    white to off-white 
solubility    DMSO: ≥20 mg/mL 
storage temp.    −20°C 
Application
MDCK cells were treated with SB 203580 to study the role of MAPK signaling in inducing hypertonicity of cell membrane and potassium depletion.16 It was used to inhibit MAPK signaling in mouse cortical neurons and human hepatocellular carcinoma cell lines.

Biochem/physiol Actions
SB 203580 is a pyridinyl imidazole that suppresses the activation of MAPKAP kinase-2 and inhibits the phosphorylation of heat shock protein (HSP) 27 in response to IL-1, cellular stresses and bacterial endotoxin in vivo. It does not inhibit JNK or p42 MAP kinase and therefore, is useful for studying the physiological roles and targets of p38 MAPK and MAPKAP kinase-2. It has been shown to induce the activation of the serine/threonine kinase Raf-1 and has been reported to inhibit cytokine production.

SB203580也写作SB 203580或SB-203580，是一种常用的p38 MAPK抑制剂。SB203580可以通透细胞，抑制p38 MAPK(p38 MAP kinase)，抑制后续MAPKAP Kinase-2和MAPKAP Kinase-3的激活。通过抑制p38 MAPK，SB203580可以有效抑制一些炎症因子(如IL-1β、TNF-α)诱导的部分信号转导。SB203580选择性抑制p38 MAPK，IC50为600 nM；对于JNK/SAPK和p44/42 MAPK(即Erk1/2)无显著的抑制作用，IC50仅为100 μM。
    SB203580分子量为377.43，分子式为C21H16N3FOS，CAS Number：152121-47-6。本产品纯度大于99%。
使用说明：
1. SB203580常见使用浓度范围为1-50μM。具体的最佳工作浓度请参考相关文献，或根据实验目的，以及所培养的特定细胞和组织，通过实验进行摸索和优化。