性能描述

form   lyophilized powder
mol wt   mol wt 21 kDa
Market Segment   Diagnostic Assay Manufacturing
storage temp.   −20°C

Application

Used to produce Fab fragments of antibodies.Also used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues.3,4

 Papain from papaya latex, has been used to digest carotid artery smooth muscle cells1. Papain is used in dissecting solutions. It is used to produce Fab fragments of antibodies.2 It is used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues.3,4

 Biochem/physiol Actions

A cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine.
pH optimum 6.0-7.0
Also hydrolyzes esters and amides.

Papain papaya latex has antifungal activity against C. albicans. It is a cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine.
. The pH optimum is 6.0-7.0
. Papain hydrolyzes esters and amides.

木瓜蛋白酶可用于细胞培养实验，在准备细胞培养液（cellculture）的第一步中被用于分离细胞。用酶处理小组织块10分钟后，就可以将连接细胞的细胞外基质打断；然后再用蛋白酶抑制剂来停止反应，防止木瓜蛋白酶进一步裂解细胞本身；最后用Pasteurpipette将组织块打散为单细胞悬浮液。
 
在免疫学中，木瓜蛋白酶可被用于将免疫球蛋白（即抗体）的Fc片段（可结晶）和Fab片段（抗原结合）切割开。