凝血酶/Thrombin/Factor IIa/人血凝血酶/凝血活素/血液凝血因子3/原激酶/凝血因子,≥2,000 NIH units/mg protein
- 产品编号：T0118
- 中文名称：凝血酶/Thrombin/Factor IIa/人血凝血酶/凝血活素/血液凝血因子3/原激酶/凝血因子
- 英文名称：Thrombin from human plasma
- 英文别名:Factor IIa
- CAS号:9002-04-4
- 纯度:≥2,000 NIH units/mg protein
- EC号:232-648-7
- MDL号:MFCD00082072
订货信息：
产品规格价格(RMB)
- T0118-100UN450
- T0118-250UN800
- T0118-1000U2000
性能描述
| 性状 |
白色或类白色的冻干块状物或粉末 |
| total impurities |
HIV and HBsAg, source material tested negative |
| storage temp. |
−20°C |
Analysis Note
The NIH assay procedure uses 0.2 ml diluted plasma (1:1 with saline) as a substrate and 0.1ml of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.
Application
Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags.
Biochem/physiol Actions
Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B.
用途：凝血酶能使纤维蛋白原转化成纤维蛋白。局部应用后作用于病灶表面的血液很快形成稳定的凝血块，用于控制毛细血管、静脉出血，或作为皮肤、组织移植物的黏合、固定剂。pH<5时失效。凝血酶对血液凝固系统的其他作用尚包括诱发血小板聚集及继发释放反应等
