性能描述

swelling    1 g swells to 4 mL (water, methanol) 
bead size    25-100 μm 
operating pH range    2 - 13 
Application
Sephadex® LH-20 is used in gel filtration chromatography, protein chromatography, gel filtration media, resins and separation media. Sephadex® LH-20 has been used to study diabetes as well as to determine differences in bioactive polyphenolic content from wild, commercial and noncommercial cultivated blackberry genotypes. Sephadex® LH-20 has also been used to produce the first report on isolation of tryptophan from the aqueous extract of lotus rhizome and demonstration of their antioxidant activities.

Legal Information
Sephadex is a registered trademark of GE Healthcare
Sephadex LH20的分离原理是根据分子大小，主要有两方面：以凝胶过滤作用为主，兼具反相分配的作用（在反相溶剂中）。因为凝胶过滤作用，所以大分子的化合物保留弱，先被洗脱下来，小分子的化合物保留强，最后出柱（具体原理可参见分子筛层析）。如果使用反相溶剂洗脱， Sephadex LH20对化合物还起反相分配的作用，所以极性大的化合物保留弱，先被洗脱下来，极性小的化合物保留强，后出柱。如果使用正相溶剂洗脱，这主要靠凝胶过滤作用来分离。