酸性磷酸酶/酸性磷酸酯酶/ACP/Acid Phosphatase/Apase,3.0-10.0 units/mg solid
- 产品编号:A0134
- 中文名称:酸性磷酸酶/酸性磷酸酯酶/ACP/Acid Phosphatase/Apase
- 英文名称:Phosphatase, Acid from potato
- 英文别名:(Orthophosphoric-monoester phosphohydrolase acid optimum)
- CAS号:9001-77-8
- 纯度:3.0-10.0 units/mg solid
- EC号:232-630-9
- MDL号:MFCD00131847
订货信息:
产品规格价格(RMB)
- A0134-10MG80
- A0134-100MG520
性能描述
性状 |
lyophilized powder |
保存温度 |
−20°C |
Biochem/physiol Actions
Acid phosphatases (APase) are a family of enzymes that non-specifically catalyze the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate at an optimum pH of 4 to 7.
APase is assayed spectrophotometrically in a 1.1 ml reaction mixture containing 41 mM citrate buffer, pH 4.8 at 37 °C, 6.9 mM p-nitrophenyl phosphate, and 0.015–0.025 unit APase.APase is soluble in cold water (0.15–0.25 unit/ml). Prepare solution immediately before use.
Storage/Stability
Store the product at –20 °C. W hen stored at –20 °C, the enzyme retains activity for at least one year.APase can lose half its activity after 30 minutes at 60 °C and 100% of its activity after two hours at 70 °C.