性能描述

Methods have been reported for the determination of glucose, hydrogen peroxide, or glucose oxidase based on the light producing oxidation of luminol catalyzed by horse radish peroxidase.1 Since the hydrogen
peroxide, which is produced is equivalent to the amount of glucose oxidized, and the light emitted is a function of the amount of hydrogen peroxide, one can estimate very small amounts of glucose compared to
spectrophotometric techniques. In the latter peroxidase and a suitable chromogenic oxygen acceptor such as o-dianisidine or o-toluidine is used. The oxidation of glucose in the presence of glucose oxidase at pH 7 produces hydrogen peroxide. The hydrogen peroxide produced is then reacted withFe(CN)63- and luminol in a basic solution to produce chemiluminescence proportional to the initial glucose concentration. After five minutes of enzyme activity,the integrated chemiluminescence signal was linear with glucose concentration in 10 ml samples over the range of 0.02-2.0 mg/ml.
This product is soluble in water (50 mg/ml), yielding a clear solution.

Solutions are sensitive to light and to the presence of metal cations, emitting light in basic solutions containing Fe3+, Cu2+, etc. Solutions are typically stable only 8-12 hrs. The sodium salt and free base
forms of luminol undergo photochemical changes resulting in the formation of a series of compounds,which are significantly inhibitory to enhanced chemiluminescence. Luminol is thermally unstable.Thus, luminol and its solutions should be protected from light and high temperature should be avoided during purification.
中文名称： 鲁米诺钠盐 　　
中文别名： 鲁米诺单钠盐;3-氨基邻苯二甲酰肼,一钠盐;3-氨基邻苯二甲酰肼一钠盐 　　
英文名称： 3-aminophthalhydrazide monosodium salt