N-Succinyl-Ala-Ala-Pro-Phe-p-nitroanilide/寡肽底物,100%
- 产品编号：S0123
- 中文名称：N-Succinyl-Ala-Ala-Pro-Phe-p-nitroanilide/寡肽底物
- 英文名称：N-Succinyl-Ala-Ala-Pro-Phe-p-nitroanilide
- 英文别名:N-Succinyl-Ala-Ala-Pro-Phe-pNA, N-Succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine 4-nitroanilide
- CAS号:70967-97-4
- 分子式:C30H36N6O9
- 分子量:624.64
- 纯度:100%
- MDL号:MFCD00077161
订货信息：
产品规格价格(RMB)
- S0123-25MG400
- S0123-100MG2600
性能描述
storage temp. -20°C
Amino Acid Sequence
N-Suc-Ala-Ala-Pro-Phe-pNA
Substrates
Colorimetric substrate for chymotrypsin, human leukocyte cathepsin G, and peptidyl prolyl isomerase.
Storage/Stability
Suc-AAPF-pNA has a shelf life of 3 years when stored desiccated at –20 °C.
Preparation Instructions
Suc-AAPF-pNA is soluble in N,N-dimethylformamide (DMF) at 25 mg/ml producing a clear, light yellow solution. It is also soluble in 5% dimethylformamide(v/v) in buffer10 and in distilled water at 4 mg/ml.
A 20 mM solution in dimethyl sulfoxide (DMSO) has been prepared and stored as a stock solution at 4 °C.Stock solutions in DMSO were diluted in 0.10 M HEPES buffer, pH 7.5, for the assay of cathepsin G.6
Suc-AAPF-pNA is soluble at 10 mM in 0.2 M Tris-HCl buffer, pH 8.0. In this buffer, a 1 mM solution of the substrate spontaneously hydrolyzes at a rate of about 0.1% per day at 4 °C.It is recommended to prepare aqueous solutions of Suc-AAPF-pNA immediately prior to use. Aqueous
solutions of the substrate were kept on ice during experiments.
