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胆碱氧化酶/Choline Oxidase,≥10 units/mg solid

  • 产品编号:C0153
  • 中文名称:胆碱氧化酶/Choline Oxidase
  • 英文名称:Choline Oxidase
  • 英文别名:Choline: oxygen 1-oxidoreductase
  • CAS号:9028-67-5
  • 纯度:≥10 units/mg solid
  • EC号:232-840-0
  • MDL号:MFCD00130787

订货信息:

产品规格价格(RMB)
  • C0153-50UN600
  • C0153-100UN(SIGMA原装)1100

性能描述

高品质,现货库存

form lyophilized powder
mol wt mol wt ~95 kDa
storage temp. −20°C

Application

Choline oxidase from Alcaligenes sp. has been used in a study to investigate a choline biosensor constructed with chitinous membrane and its application in measuring cholinesterase inhibitory activities. Choline oxidase from Alcaligenes sp. has also been used in spectroscopic studies on the photoreaction of choline oxidase with covalently bound flavin.

Physical properties

Isoelectric point: 4.1 ± 0.1
Michaelis constants: 2.84 x 10¯3M (Choline), 5.33 x 10¯3M (Betaine aldehyde)
Structure: One mol of FAD is covalently bound to mol of the enzyme
Inhibitors: p-Chloromercuribenzoate, Cu++, Co++, Hg++, Ag+
Optimum pH: 8.0 – 8.5
Optimum temp: 40 – 45°C
pH Stability: pH 7.0 – 9.0 (30°C, 2hr)
Thermal stability: Below 37°C (pH 7.5, 10min)

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