性能描述

Biochem/physiol Actions
Mode of Action: Inhibits DNA synthesis by inhibiting the enzyme Topoisomerase II.
Antimicrobial spectrum: Gram-positive bacterial.
Novobiocin inhibits DNA synthesis by inhibiting bacterial DNA gyrase targeting the GyrB subunit of the enzyme involved in energy transduction. Novobiocin acts as a competitive inhibitors of the ATPase reaction catalysed by GyrB. It is also a Hsp90 inhibitor 3. Antimicrobial spectrum: Gram-positive bacterial.

Application
For the production of positively supercoiled plasmid DNA.Inhibitor of bacterial DNA gyrase and eukaryotic DNA topoisomerase.Inhibitor of retrovirus RNA-dependent DNA-polymerase.
Novobiocin is used to study heat shock protein inhibition and to produce positively supercoiled plasmid DNA.

对革兰阳性球菌和杆菌（包括厌氧菌）的抗菌作用基本与青霉素相同，对粪肠球菌的作用较青霉素强。革兰阴性细菌中脑膜炎奈瑟菌、淋病奈瑟菌、流感嗜血杆菌、百日咳杆菌、布氏杆菌、奇异变形杆菌、沙门菌等皆对本品敏感。某些大肠埃希菌及某些志贺菌属也对本品敏感，但多数志贺菌属对本品耐药，其他肠杆菌科细菌、铜绿假单胞菌、脆弱拟杆菌等对本品耐药。本品对军团菌和胎儿弯曲杆菌有一定抗菌作用。本品的作用机制是抑制细菌细胞壁的合成，使细菌迅速破裂溶解。