噻唑蓝/溴化噻唑蓝四氮唑/噻唑兰/MTT,≥97.5% (HPLC)
- 产品编号：M0105
- 中文名称：噻唑蓝/溴化噻唑蓝四氮唑/噻唑兰/MTT
- 英文名称：Thiazolyl Blue Tetrazolium Bromide
- 英文别名:MTT, Methylthiazolyldiphenyl-tetrazolium bromide,3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide
- CAS号:298-93-1
- 分子式:C18H16BrN5S
- 分子量:414.32
- 纯度:≥97.5% (HPLC)
- EC号:206-069-5
- MDL号:MFCD00011964
订货信息：
产品规格价格(RMB)
- M0105-1G100
- M0105-5G350
- M0105-25G1500
- M0105-100G5000
性能描述
| 试剂级别 |
生化试剂，生物染料 |
| 试剂触怒 |
≥97.5% (HPLC) |
| form |
powder |
| mp |
195 °C (dec.)(lit.) |
| solubility |
H2O: soluble5 mg/mL |
| suitability |
suitable for cell culture |
| |
suitable for insect cell culture |
| 保存温度 |
2-8°C |
Application
Use as a colorimetric metabolic activity indicator in cell viability assays.
Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation.MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent6 and for the detection of NAD.7 ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used.8 MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.9
MTT主要有两个用途：
1．药物（也包括其他处理方式如放射线照射）对体外培养的细胞毒性的测定；
2．细胞增殖及细胞活性测定。 检测原理为活细胞线粒体中的琥珀酸脱氢酶能使外源性MTT还原为水不溶性的蓝紫色结晶甲瓒（Formazan）并沉积在细胞中，而死细胞无此功能。二甲基亚砜（DMSO）能溶解细胞中的甲瓒，用酶标仪在490nm波长处测定其光吸收值，在一定细胞数范围内，MTT结晶形成的量与细胞数成正比。根据测得的吸光度值（OD值），来判断活细胞数量，OD值越大，细胞活性越强（如果是测药物毒性，则表示药物毒性越小）。
性状溶解性：
黄色或橙黄色粉末，熔点：195℃(分解）。微溶于甲醇中，但难溶于水或乙醇中。用醚、丙酮或乙酸乙酯也不能将其完全溶解。195℃溶于水或DMSO。常配制成5mg/ml的储备液。
【中文名称】噻唑蓝
【中文别名】噻唑兰，溴化噻唑蓝四氮唑，3-(4,5-二甲基-2-噻唑)-2,5-二苯基溴化四氮唑噻唑蓝，四甲基偶氮唑盐
【英文名称】3-(4,5-Dimethyl-2-Thiazolyl)-2,5-Diphenyl Tetrazolium Bromide；Thiazolyl Blue Tetrazolium Bromide；MTT
