性能描述

试剂级别    生化试剂 
form    crystalline 
potency    1.5-2.0 units per mg 
solubility    H2O: soluble20 mg/mL, clear, colorless 
cation traces    Cu: ≤0.10% 
suitability    suitable for cell culture 
storage temp.    2-8°C 
Application
Recommended for use as a selection agent at 10-100 μg/ml.

Bleomycin is used as a transformed cell selection agent, especially for plant transformants. Recommended for use at 10-100 g/ml. It is also used to induce pulmonary fibrosis and epithelial cell apoptosis. 1,2,3

Biochem/physiol Actions
An antineoplastic antibiotic isolated from Streptomyces verticillus. Binds to DNA, inhibits DNA synthesis and causes DNA scissions at specific base sequences. Needs to bind oxygen and a metal ion such as copper or iron to cleave DNA. Highly selective cleavage of RNA. Inducer and regulator of apoptosis in a variety of cells. Inhibits tumor angiogenesis.

博来霉素含有若干性质近似的组分，为弱碱性物质。易溶于水、甲醇，微溶于乙醇，几乎不溶于丙酮、乙酸乙酯、乙酸丁酯、乙醚。性状为白色至淡黄色粉末。

博来霉素与铁的复合物嵌入DNA，引起DNA单链和双链断裂。它不引起RNA链断裂。作用的第一步是本品的二噻cuo环嵌入DNA的G-C碱基对之间，同时末端三肽氨 基酸的正电荷和DNA磷酸基作用，使其解链。作用的第二步是本品与铁的复合物导致超氧或羟自由基的生成，引起DNA链断裂。