低电渗琼脂糖/低电内渗琼脂糖/low EEO Agarose,~100%
- 产品编号:A0203
- 中文名称:低电渗琼脂糖/低电内渗琼脂糖/low EEO Agarose
- 英文名称:Agarose(Type I-A, low EEO)
- CAS号:9012-36-6
- 纯度:~100%
- EC号:232-731-8
- MDL号:MFCD00081294
性能描述
说明:该产品为SIGMA分装试剂(原装货号A0169-500G)。
type Type I-A
impurities ≤7% water
ash ≤0.5%
turbidity ≤4 NTU (1.5% gel)
EEO 0.09-0.13
transition temp gel point 36 °C ±1.5 °C
gel strength ≥1200 g/cm2 (1% gel)
anion traces sulfate (SO42-): ≤0.20%
Analysis Note
The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.