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溶壁酶/细胞溶解酶/Lyticase/Lysing Enzymes,≥200 units/mg solid (Sigma)

  • 产品编号:L0206
  • 中文名称:溶壁酶/细胞溶解酶/Lyticase/Lysing Enzymes
  • 英文名称:Lyticase
  • 英文别名:Lysing Enzymes
  • CAS号:37340-57-1
  • 纯度:≥200 units/mg solid (Sigma)
  • MDL号:MFCD00131560

订货信息:

产品规格价格(RMB)
  • L0206-5KU(SIGMA分)260
  • L0206-1G(国产)300

性能描述

form    lyophilized powder 
storage temp.    −20°C 
Biochem/physiol Actions
Lyticase hydrolyzes poly-β(1→3)-glucose such as yeast cell wall glucan.

Application
Yeast cells are difficult to disrupt because the cell walls may form capsules or resistant spores. DNA can be extracted from yeast by using lysing enzymes such as lyticase, chitinase, zymolase, and gluculase to induce partial spheroplast formation; spheroplasts are subsequently lysed to release DNA. Lyticase is preferred to digest cell walls of yeast and generate spheroplasts from fungi for transformation.
Reported to be useful for lysis of Ashbya, Candida, Debaryomyces, Eremothecium, Endomyces, Hansenula, Hanseniaspora, Kloeckera, Kluyveromyces, Lipomyces, Metschikowia, Pichia, Pullularia, Torulopsis, Saccharomyces, Saccharomycopsis, Saccharomycodes, and Schwanniomyces species.

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